tag:blogger.com,1999:blog-14688252.post3460720123669061205..comments2024-02-16T10:17:12.310+01:00Comments on YOKOFAKUN: Rare Disease: How-to ?Pierre Lindenbaumhttp://www.blogger.com/profile/13765837643388003852noreply@blogger.comBlogger3125tag:blogger.com,1999:blog-14688252.post-87056876465772058132009-05-19T21:20:00.000+02:002009-05-19T21:20:00.000+02:00What do you know about the effect of the disease ?...What do you know about the effect of the disease ? Symptoms, metabolic alterations, physiological effects … These types of information can provide idea about the target of your gene.Unknownhttps://www.blogger.com/profile/18424004068235159407noreply@blogger.comtag:blogger.com,1999:blog-14688252.post-64013211976078843532009-05-18T23:20:00.000+02:002009-05-18T23:20:00.000+02:00Thanks Lucas, This protein is not a transcription ...Thanks Lucas, This protein is not a transcription factor and has no ortholog is yeast. But TAP-tagging would be a good start !Pierre Lindenbaumhttps://www.blogger.com/profile/13765837643388003852noreply@blogger.comtag:blogger.com,1999:blog-14688252.post-45724568138104904862009-05-18T22:48:00.000+02:002009-05-18T22:48:00.000+02:00In the case of Y2H, you would definitely need to d...In the case of Y2H, you would definitely need to do a cellular localization assay (fluorescent markers) to see if the interactors localize to the same organelle/to the cytoplasm. Other interaction assays include TAP-tagging, coupled to mass spectrometry and the like..<br />If it's a transcription factor you could do something like ChIP-sequencing to see to which DNA sequences it binds.<br />Lucas Brouwershttps://www.blogger.com/profile/15192035237302508309noreply@blogger.com